INVESTIGATIONS OF ADSORPTION OF UNSATURATED FATTY ACID METHYL ESTERS ON SILICIC ACID-SILVER NITRATE

被引：17

作者：

STEIN, RA

SLAWSON, V

机构：

[1] Department of Biological Chemistry, School of Medicine, University of California Los Angeles, Los Angeles

来源：

ANALYTICAL CHEMISTRY | 1968年 / 40卷 / 13期

关键词：

D O I：

10.1021/ac60269a006

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Silicic acid-silver nitrate adsorbent was prepared by the addition of ethyl ether to an acetonitrile solution of silver nitrate on a silicic acid chromatographic column. Partition isotherms were measured for methyl esters of cisunsaturated fatty acids between the adsorbent and cyclohexeneether-pentane mixtures, and solvents that gave 40-80% of a solute in solution eluted the solute satisfactorily from a chromatographic column. A scheme was developed that gave good separations by elution chromatography of a mixture of methyl esters of fatty acids containing from 0 to 6 methylene-inter-rupted cis-double bonds. Competition for adsorption sites between methyl oleate and methyl docosahexaenoate was dependent upon the concentration of each ester. Electron micrographs of the support suggest that the silver nitrate is distributed as small crystals within the silicic acid granules. © 1968, American Chemical Society. All rights reserved.

引用

页码：2017 / &

共 10 条

[1] QUANTITATIVE SEPARATIONS OF HIGHER FATTY ACID METHYL ESTERS BY ADSORPTION CHROMATOGRAPHY ON SILICA IMPREGNATED WITH WILVER NITRATE [J].