35CL-NMR STUDIES ON ZINC-PYRUVATE KINASE COMPLEXES

被引：25

作者：

COTTAM, GL

WARD, RL

机构：

[1] Veterans Administration Hospital, Dallas

[2] Lawrence Radiation Laboratory, Livermore

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 132卷 / 01期

关键词：

D O I：

10.1016/0003-9861(69)90367-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

35Cl NMR is used to study the interaction of zinc with pyruvate kinase. The number of zinc ions bound per molecule of pyruvate kinase is determined to be 4.17 ± 0.2 and all four sites apparently have the same association constant of 0.81 ± 0.07 × 105 m-1. Decreases in the 35Cl-NMR line width are observed upon addition of HCO3-, ATP, or ADP to the zinc-pyruvate kinase binary complex. These effects could be explained by ternary complex formation or conformational changes effecting the environment of the bound zinc ion. © 1969.

引用

页码：308 / &

共 18 条

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