CDNA-ENCODING THE MAJOR MITE ALLERGEN DER-F-II

被引：78

作者：

TRUDINGER, M ^{[1
]}

CHUA, KY ^{[1
]}

THOMAS, WR ^{[1
]}

机构：

[1] PRINCESS MARGARET HOSP,WESTERN AUSTRALIAN RES INST CHILD HLTH,GPO BOX D184,PERTH,WA 6001,AUSTRALIA

来源：

CLINICAL AND EXPERIMENTAL ALLERGY | 1991年 / 21卷 / 01期

关键词：

D O I：

10.1111/j.1365-2222.1991.tb00801.x

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

cDNA encoding the major house dust mite allergen Der f II from Dermatophagoides farinae was amplified using the polymerase chain reaction and cloned into E. coli. It encoded a 129-residue protein with a calculated molecular weight of 14021 D and had the expected high homology (88%) with Der p II including the absence of N-glycosylation sites and conserved cysteine residues. These results are consistent with the high degree of antibody crossreactivity and may help identify the differences in T-cell epitopes revealed for these molecules so far.

引用

收藏

页码：33 / 37

页数：5

相关论文

共 20 条

[1]

BAKER E, 1983, BIOL MACROMOL, V3, P313

[2] ISOLATION OF CDNA CODING FOR THE MAJOR MITE ALLERGEN DER P-II BY IGE PLAQUE IMMUNOASSAY [J].

CHUA, KY ;

DOYLE, CR ;

SIMPSON, RJ ;

TURNER, KJ ;

STEWART, GA ;

THOMAS, WR .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1990, 91 (02) :118-123

[3] SEQUENCE-ANALYSIS OF CDNA CODING FOR A MAJOR HOUSE DUST MITE ALLERGEN, DER-P-1 HOMOLOGY WITH CYSTEINE PROTEASES [J].

CHUA, KY ;

STEWART, GA ;

THOMAS, WR ;

SIMPSON, RJ ;

DILWORTH, RJ ;

PLOZZA, TM ;

TURNER, KJ .

JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 167 (01) :175-182

[4]

DILWORTH RJ, 1990, CLIN EXP ALLERGY, V21, P25

[5] USE OF THE DNA-POLYMERASE CHAIN-REACTION FOR HOMOLOGY PROBING - ISOLATION OF PARTIAL CDNA OR GENOMIC CLONES ENCODING THE IRON SULFUR PROTEIN OF SUCCINATE-DEHYDROGENASE FROM SEVERAL SPECIES [J].

GOULD, SJ ;

SUBRAMANI, S ;

SCHEFFLER, IE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (06) :1934-1938

[6] A SIMPLE AND VERY EFFICIENT METHOD FOR GENERATING CDNA LIBRARIES [J].

GUBLER, U ;

HOFFMAN, BJ .

GENE, 1983, 25 (2-3) :263-269

[7] ANTIGENIC AND STRUCTURAL-ANALYSIS OF GROUP-II ALLERGENS (DER-F-II AND DER-P-II) FROM HOUSE DUST MITES (DERMATOPHAGOIDES SPP) [J].

HEYMANN, PW ;

CHAPMAN, MD ;

AALBERSE, RC ;

FOX, JW ;

PLATTSMILLS, TAE .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1989, 83 (06) :1055-1067

[8] PREDICTION OF PROTEIN ANTIGENIC DETERMINANTS FROM AMINO-ACID-SEQUENCES [J].

HOPP, TP ;

WOODS, KR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (06) :3824-3828

[9]

HUYNH TV, 1985, DNA CLONING, V2, P47

[10] HLA CLASS-II RESTRICTION SPECIFICITY OF DERMATOPHAGOIDES SPP REACTIVE LYMPHOCYTE-T CLONES THAT SUPPORT IGE SYNTHESIS [J].

LAMB, JR ;

KAY, AB ;

OHEHIR, RE .

CLINICAL AND EXPERIMENTAL ALLERGY, 1989, 19 (04) :389-393