IMMUNOHISTOCHEMICAL DETECTION OF DNA ALKYLATION ADDUCTS IN RAT AND HAMSTER LIVER AFTER TREATMENT WITH DIMETHYLNITROSAMINE

被引：18

作者：

ASAMOTO, M ^{[1
]}

MIKHEEV, AM ^{[1
]}

JIANG, YZ ^{[1
]}

WILD, CP ^{[1
]}

HALL, J ^{[1
]}

MONTESANO, R ^{[1
]}

机构：

[1] INT AGCY RES CANC, MECHANISMS CARCINOGENESIS UNIT, F-69008 LYONS, FRANCE

来源：

EXPERIMENTAL PATHOLOGY | 1991年 / 41卷 / 02期

关键词：

DNA ALKYLATION; LIVER; DIMETHYLNITROSAMINE; IMMUNOHISTOCHEMISTRY; DNA;

D O I：

10.1016/S0232-1513(11)80004-6

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Monoclonal and polyclonal antibodies specific for methylation adducts have been applied in an immunohistochemical study of DNA damage in rat and hamster liver following exposure to dimethylnitrosamine. The approach was validated, for frozen and paraffin-embedded sections, by comparison with biochemical data on adduct levels in whole tissues or specific cell populations in the same experimental systems. The potential application of this method to human exposure assessment is discussed.

引用

页码：71 / 78

页数：8

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