SELECTIVE EXTRACTION AND QUANTITATION OF POLYOXYETHYLENE DETERGENTS AND ITS APPLICATION IN PROTEIN DETERMINATION

被引：14

作者：

TERSTAPPEN, GC

KULA, MR

机构：

[1] Institut für Enzymtechnologie der Heinrich-Heine-Uni-versität Düsseldorf, Jülich, D-5170, Forschungszentrum Jülich

来源：

ANALYTICAL LETTERS | 1990年 / 23卷 / 12期

关键词：

NONIONIC DETERGENTS; HPLC; DCE-EXTRACTION; PROTEIN DETERMINATION;

D O I：

10.1080/00032719008052558

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple, rapid and selective method for the nearly quantitative extraction of high amounts (10% w/v) of polyoxyethylene nonionic detergents like Triton X-100R is presented based on solvent extraction with 1,2-dichloroethane. Protein determination after extraction of protein containing solutions can be accomplished using the Bradford assay 1 or other standard methods. High protein recoveries can be obtained. The detergent analysis by HPLC using reversed-phases is not disturbed in the presence of proteins and therefore provides a fast and elegant method for the exact quantitation of nonionic detergents.

引用

页码：2175 / 2193

页数：19

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