CONSTRUCTION OF PHYSICAL AND GENETIC MAPS OF CHLAMYDIA-TRACHOMATIS SEROVAR-L2 BY PULSED-FIELD GEL-ELECTROPHORESIS

We constructed the physical map of Chlamydia trachomatis serovar L2 by using three restriction endonucleases, NotI (GC\GGCCGC), SgrAI (C(A/G)\CCGG(T/G)G), and Sse83871 (CCTGCA\GG), and we analyzed the fragments by pulsed-field gel electrophoresis. A total of 25 restriction endonuclease sites and 13 genes and/or operons were located on the map. The genome size was determined to be 1,045 kb. Neither highly transcribed chiamydia genes nor developmental cycle-specific genes were clustered on the genome.