ISOLATION OF A HUMAN YAC CONTIG ENCOMPASSING A CLUSTER OF UGT2 GENES AND ITS REGIONAL LOCALIZATION TO CHROMOSOME 4Q13

被引：53

作者：

MONAGHAN, G

CLARKE, DJ

POVEY, S

SEE, CG

BOXER, M

BURCHELL, B

机构：

[1] UNIV DUNDEE, NINEWELLS HOSP & MED SCH, DEPT BIOCHEM MED, DUNDEE DD1 9SY, SCOTLAND

[2] UNIV DUNDEE, NINEWELLS HOSP & MED SCH, DEPT PATHOL, DUNDEE DD1 9SY, SCOTLAND

[3] UNIV LONDON UNIV COLL, DEPT GENET & BIOMETRY, GALTON LAB, LONDON NW1 2HE, ENGLAND

[4] UNIV LONDON UNIV COLL, MRC, HUMAN BIOCHEM GENET UNIT, LONDON NW1 2HE, ENGLAND

来源：

GENOMICS | 1994年 / 23卷 / 02期

基金：

英国惠康基金;

关键词：

D O I：

10.1006/geno.1994.1531

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Previously we mapped the gene encoding a human bile acid UDP-glucuronosyltransferase (UGT2B4) to chromosome 4. Here we report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell hybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes was isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15. Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13. (C) 1994 Academic Press, Inc.

引用

页码：496 / 499

页数：4

共 22 条

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