EXPRESSION OF THE IL-2 RECEPTOR-GAMMA CHAIN ON VARIOUS POPULATIONS IN HUMAN PERIPHERAL-BLOOD

被引：106

作者：

ISHII, N ^{[1
]}

TAKESHITA, T ^{[1
]}

KIMURA, Y ^{[1
]}

TADA, K ^{[1
]}

KONDO, M ^{[1
]}

NAKAMURA, M ^{[1
]}

SUGAMURA, K ^{[1
]}

机构：

[1] TOHOKU UNIV,SCH MED,DEPT MICROBIOL,AOBA KU,SENDAI,MIYAGI 98077,JAPAN

来源：

INTERNATIONAL IMMUNOLOGY | 1994年 / 6卷 / 08期

基金：

日本科学技术振兴机构;

关键词：

IL-2; IL-2 RECEPTOR GAMMA CHAIN; MONOCLONAL ANTIBODY; PERIPHERAL BLOOD;

D O I：

10.1093/intimm/6.8.1273

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have established two rat mAbs, TUGh4 and TUGh5, specific for the human gamma chain of the IL-2 receptor (IL-2R), which is known to be shared among receptors for IL-2, IL-4 and IL-7. The antibodies bound to cell lines transfected with the human gamma chain gene but not to their parental cell lines, and precipitated 65 - 70 and 80 - 90 kDa cell surface molecules from lysates of human T cells surface-labeled with (NaI)-I-125 and chemically cross-linked with [I-125]IL-2 respectively. Flow cytometry with TUGh4 and TUGh5 detected the gamma chain in a wide variety of peripheral blood cell populations including CD4(+) T cells, CD8(+) T cells, CD20(+) B cells, CD56(+) natural killer cells, CD14(+) monocytes and granulocytes, contrasting with expression of the alpha and beta chains of IL-2R.

引用

页码：1273 / 1277

页数：5

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