METABOLISM OF 2,4,4'-TRICHLOROBIPHENYL BY ACINETOBACTER SP P6

Metabolism of 2, 4, 4'-trichIorobipheny] by Acinetobacter sp. strain P6 has been studied. When the incubation was carried out without shaking at 15°C, two isomeric monohydroxy compounds, a dihydrodiol compound, a dihydroxy compound, a meta-cleaved yellow compound and a dichlorobenzoic acid were detected by combined gas liquid chromatograph-mass spectrometry. As an additional metabolite, dichlorodihydroxy biphenyl, a dechlorination-hydroxylation product, was also detected. When the incubation mixture was shaken at 30°C, a meta-cleaved yellow compound was readily produced and predominantly accumulated in the reaction mixture upon further incubation. The major pathway of 2, 4, 4'-trichloro-biphenyl by Acinetobacter sp. P6 was considered to proceed oxidatively via 2', 3'-dihydro-2', 3 diol compound, concomitant dehydrogenated 2', 3'-dihydroxy compound and then the l', 2'-meta-cleaved yellow compound, i.e., 3-chloro-2-hydroxy-6-oxo-6(2, 4-dichlorophenyl)hexa-2, 4-dienoic acid. © 1979 Taylor and Francis Group, LLC.