LOCALIZATION OF MUTAROTASE IN RAT-KIDNEY

被引：7

作者：

BABA, S ^{[1
]}

ARIMOTO, Y ^{[1
]}

YOSHIKAWA, D ^{[1
]}

TOYODA, Y ^{[1
]}

MIWA, I ^{[1
]}

OKUDA, J ^{[1
]}

机构：

[1] MEIJO UNIV,FAC PHARM,DEPT CLIN BIOCHEM,TEMPAKU KU,NAGOYA,AICHI 468,JAPAN

来源：

EXPERIENTIA | 1979年 / 35卷 / 08期

关键词：

D O I：

10.1007/BF01949964

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The localization of mutarotase in rat kidney was investigated by fluorescein-labelled and peroxidase-labelled antibody techniques, and by method of isolation of the nuclei and cytoplasm in non-aqueous solvents. In these immunohistochemical studies, mutarotase was almost exclusively recognized in the nuclei of epithelial cells of renal tubules and glomeruli in rat. The specific activity of mutarotase was found to be 1.5 times higher in the nuclei (122 units/g dry wt) than that in the cytoplasm (80 units/g dry wt) isolated with non-aqueous solvents. These results suggest that mutarotase may be involved in the metabolism of D-glucose in nuclei. © 1979 Birkhäuser Verlag.

引用

页码：1094 / 1097

页数：4

共 20 条

[1]

AVRAMEAS S, 1976, METH ENZYMOL, V44, P713

[2] DISTRIBUTION OF A MUTAROTASE ACTIVITY IN RAT TISSUES AND POSSIBLE FUNCTION IN ACTIVE TRANSPORT OF SUGARS [J].

BAILEY, JM ;

PENTCHEV, PG ;

WOO, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 94 (01) :124-&

[3] STUDIES ON MUTAROTASES .5. SERUM MUTAROTASE LEVELS IN RENAL DISEASE [J].

BAILEY, JM ;

FISHMAN, PH ;

MULHERN, S ;

MURRAY, R .

CLINICAL BIOCHEMISTRY, 1970, 3 (01) :11-&

[4]

BAILEY JM, 1973, ADV CHEM SER, V117, P264

[5] MUTAROTASE (ALDOSE-1-EPIMERASE) CATALYZED ANOMERIZATION OF GLUCOSE-6-PHOSPHATE [J].

GERNERT, E ;

KESTON, AS .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1974, 161 (02) :420-425

[6]

KESTON AS, 1964, J BIOL CHEM, V239, P3241

[7] OCCURRENCE OF MUTAROTASE IN ANIMALS - ITS PROPOSED RELATIONSHIP TO TRANSPORT AND REABSORPTION OF SUGARS AND INSULIN [J].

KESTON, AS .

SCIENCE, 1954, 120 (3113) :355-356

[8] DPN PYROPHOSPHORYLASE [J].

KORNBERG, A .

METHODS IN ENZYMOLOGY, 1955, 2 :670-672

[9] RAPID POLAROGRAPHIC MUTAROTASE ASSAY WITH BETA-D-GLUCOSE OXIDASE [J].

MIWA, I .

ANALYTICAL BIOCHEMISTRY, 1972, 45 (02) :441-&

[10]

MIWA I, 1974, CHEM PHARM BULL, V22, P2337

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