HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF LOXOPROFEN AND ITS DIASTEREOMERIC ALCOHOL METABOLITES IN BIOLOGICAL-FLUIDS BY FLUORESCENCE LABELING WITH 4-BROMOMETHYL-6,7-METHYLENEDIOXYCOUMARIN

被引：24

作者：

NAGANUMA, H

KAWAHARA, Y

机构：

[1] Product Development Laboratories, Sankyo Co., Ltd., Shinagawa-ku, Tokyo, 140, 2-58

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 530卷 / 02期

关键词：

D O I：

10.1016/S0378-4347(00)82341-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and sensitive high-performance liquid chromatographic procedure to determine loxoprofen and its diastereomeric alcohol metabolites in biological specimens is described. The analysis involves liquid-liquid extraction with benzene, pre-column derivatization with a highly fluorogenic reagent, 4-bromomethyl-6,7-methylenedioxycoumarin (BrMDC) and subsequent separation on a reversed-phase column. Loxoprofen, its pharmacologically active metabolite, trans-alcohol, and less active cis-alcohol were completely separated within 20 min with a mobile phase of 55% of aqueous acetonitrile containing acetic acid. Any endogenous substances do not interfere in the analysis of either plasma or urine samples. The quantitation limit was 0.01 μg/ml for human plasma and 0.05 μg/ml for urine. The method was applied to a pharmacokinetic study in healthy human subjects who had received 60 mg of loxoprofen sodium. © 1990.

引用

页码：387 / 396

页数：10

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