ANDROGEN METABOLISM BY PORCINE GRANULOSA-CELLS DURING THE PROCESS OF LUTEINIZATION INVITRO - IDENTIFICATION OF 19-OIC-ANDROSTENEDIONE AS A MAJOR METABOLITE AND POSSIBLE PRECURSOR FOR THE FORMATION OF C-18 NEUTRAL STEROIDS

The present studies were conducted to define the pathway(s) by which androstenedione is metabolized in porcine granulosa cells (pGC) and determine whether metabolism of this steroid is affected by in vitro luteinization. pGC isolated from large preovulatory follicles were cultured for up to 2 days in the presence of 5-mu-M unlabeled or [4-C-14]-labeled androstenedione. Metabolism of androstenedione was assessed by HPLC, using in-line liquid scintillation detection. Metabolite identification was confirmed by gas chromatography-mass spectrometry of HPLC fractions isolated from medium conditioned by granulosa cells (pGCCM) cultured for 48 h in the presence of unlabeled androstenedione. The metabolites identified were 19-oic-androstenedione (3,17-dioxo-4-androsten-19-oic acid), 19-hydroxytestosterone, 19-hydroxyandrostenedione, 19-nor-testosterone, an estrenolone of as yet unproven stereoisomerism, 5(10)-estrene-3-beta, 17-beta-diol, 17-beta-estradiol, testosterone, and 19-nor-androstenedione. Results indicate that 19-nor-androstenedione is artifactually derived from 19-oic-androstenedione as a result of degradation in storage and during isolation. After metabolite identification, studies of the time course of androstenedione metabolism by pGC during in vitro luteinization were conducted. 17-beta-Estradiol and 19-oic-androstenedione were the predominant metabolites, and accumulation of these steroids was virtually identical. Production of these metabolites was maximal during the first 12 h of culture. The accumulation of 5(10)-estrene-3-beta, 17-beta-diol and 19-nor-testosterone was maximal at 48 h of culture, with 5(10)-estrene-3-beta, 17-beta-diol consistently accumulating in greater concentrations than 19-nor-testosterone. Aromatase activity of pGC was negligible from 36-48 h of culture, as demonstrated by minimal accumulation of 17-beta-estradiol during this period of culture. The accumulation of 19-oic-androstenedione, 5(10)-estrene-3-beta, 17-beta-diol, and 19-nor-testosterone was also negligible during this latter time period, suggesting that their formation is associated with aromatase. From these results, pGC from preovulatory follicles undergoing luteinization in vitro lose the ability to convert androstenedione to estrogens. The formation of 19-oic-androstenedione, shown here for the first time, parallels the formation of 17-beta-estradiol, and this acidic steroid is proposed to be a product C-18 neutral steroids from exogenous androstenedione. The production of these steriods requires an active aromatase to produce their immediate precusor, which is here hypothesized to be 19-oic-androstenedione. However, their maximal production does not commence until aromatase activity has declined, and it is hypothesized that their production depends on modifications in steroid metabolism associated with luteinization.