LOCALIZATION AND COMPOSITION OF LIPIDS IN NEONATAL MOUSE STRATUM GRANULOSUM AND STRATUM-CORNEUM

被引:212
作者
ELIAS, PM
BROWN, BE
FRITSCH, P
GOERKE, J
GRAY, GM
WHITE, RJ
机构
[1] UNIV CALIF SAN FRANCISCO, SCH MED, DEPT DERMATOL, SAN FRANCISCO, CA 94143 USA
[2] UNIV CALIF SAN FRANCISCO, SCH MED, DEPT PHYSIOL, SAN FRANCISCO, CA 94143 USA
[3] UNIV CALIF SAN FRANCISCO, SCH MED, CARDIOVASC RES INST, SAN FRANCISCO, CA 94143 USA
[4] UNIV BIRMINGHAM, MRC, EXPTL PATHOL SKIN UNIT, BIRMINGHAM B15 2TT, W MIDLANDS, ENGLAND
[5] UNIV INNSBRUCK, SKIN CLIN DERMATOL & SYPHILOL, A-6020 INNSBRUCK, AUSTRIA
关键词
D O I
10.1111/1523-1747.ep12550377
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Recent cytochemical and freeze-fracture experiments indicate that intercellular lipids, derived from the secreted contents of epidermal lamellar bodies, may be important for the permeability barrier in skin. During keratinization extensive changes occur in the morphology and histochemistry of intercellular lamellae that coincide with profound alterations in the lipid composition of the stratum granulosum and stratum corneum. In the present study we have separated stratum granulosum from stratum corneum and measured their lipid compositions. In the stratum granulosum polar and neutral lipids are almost equally abundant, and are found cytochemically both between and within cells. Lamellar bodies examined by cytochemical techniques contain free sterols and neutral sugars, suggesting the presence of glycosphingolipids, but little phospholipid. On the other hand, the stratum corneum contains predominantly neutral lipids and sphingolipids which are found primarily in cell membrane regions. Based upon these data, we suggest that lamellar bodies secrete glycolipids and free sterols, which are then extensively metabolized to free fatty acids, ceramides, and sterol esters. Together with some remaining free sterols these coalesce to form the broad bilayers of the stratum corneum interstices. These hydrophobic lipids are thus ideally situated and suited to function as the principal epidermal permeability barrier.
引用
收藏
页码:339 / 348
页数:10
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