MAPPING BY MULTIFRAGMENT CLONING IN-VIVO

被引：20

作者：

MARYKWAS, DL

PASSMORE, SE

机构：

[1] Dept. of Molec. and Cellular Biology, Harvard University, Cambridge

[2] Department of Molecular Biology, BioInsight, Inc., Cambridge

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 25期

关键词：

RECOMBINATION; RECOMBINANT DNA; 2-HYBRID SYSTEM;

D O I：

10.1073/pnas.92.25.11701

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An efficient method for mapping mutations is described in which hybrid genes, derived partly from mutant and partly from wild-type DNA, are obtained in vivo by homologous recombination of multiple fragments. The recombinants are formed in a strain in which their phenotypes are immediately apparent. This method was developed to identify changes that disrupt protein-protein interactions demonstrable by the two-hybrid system in yeast. However, it can be extended to any system where recombination is possible, provided an assay is available to distinguish between mutant and wild-type phenotypes.

引用

页码：11701 / 11705

页数：5

共 21 条

[1] THE 2-HYBRID SYSTEM - A METHOD TO IDENTIFY AND CLONE GENES FOR PROTEINS THAT INTERACT WITH A PROTEIN OF INTEREST [J].