BASE ANALYSIS OF RNA - IMPLICATIONS FOR RNA ANALYSIS OF EFFECTS OF HEATING PURINES AND PYRIMIDINE NUCLEOTIDES WITH 1 N HCL AT 100 DEGREES FOR ONE HOUR

被引：2

作者：

SCHEIN, AH

DANGELO, C

机构：

[1] Department of Biochemistry, College of Medicine, University of Vermont, Burlington

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 29卷 / 02期

关键词：

D O I：

10.1016/0003-2697(69)90316-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Heating (a) adenine-8-14C, (b) guanine-8-14C, (c) UMP-2-14C, and (d) CMP-2-14C with 1 N HCl at 100° for 60 min, conditions normally employed to hydrolyze RNA for base analysis, yielded the following results: (a) 5% destruction of A, 2 5 of which could be accounted for as Hx, (b) G unaffected, recovery 100%, (c) 3% UMP disappearance, the major portion converted to UR, (d) 9% CMP disappearance, half of which could be isolated as derivatives-UMP and CR. Implications for RNA analysis are discussed. © 1969.

引用

页码：323 / &

共 17 条

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[2]

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[3]

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[4]

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