SPECIFIC ENZYMATIC ASSAY FOR D-GLUCARATE IN HUMAN SERUM

被引：14

作者：

BLUMENTHAL, HJ

LUCUTA, VL

BLUMENTHAL, DC

机构：

[1] Department of Microbiology, Loyola University of Chicago, Stritch School of Medicine, Maywood

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 185卷 / 02期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0003-2697(90)90294-J

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and specific spectrophotometric assay was developed to determine levels of d-glucarate in human serum. This assay makes use of the Escherichia coli glucarate catabolic enzymes d-glucarate dehydrase, α-keto-β-deoxy-d-glucarate aldolase, and tartronate semialdehyde (TSA) reductase, to convert d-glucarate to equimolar quantities of pyruvate and TSA. In a one-tube reaction that included NADH, lactate dehydrogenase, and the three E. coli enzymes, 1 μmol of d-glucarate was quantitatively converted to 1 μmol each of d-glycerate and l-lactate with concomitant utilization of 2 μmol of NADH. Using this method, d-glucarate in serum was measured, along with quantitative recovery of authentic d-glucarate from duplicate serum samples to which it had been added. Glucarate is a major serum organic acid, approximating blood pyruvate levels previously determined by others. © 1990.

引用

页码：286 / 293

页数：8

共 25 条

[1] EVIDENCE FOR FUNCTION OF D-GLUCARIC ACID AS AN INDICATOR FOR DRUG INDUCED ENHANCED METABOLISM THROUGH GLUCURONIC ACID PATHWAY IN MAN [J].