COMPOSITION AND STRUCTURAL FEATURES OF CELL-WALL POLYSACCHARIDES FROM TOMATO FRUITS

被引:159
作者
SEYMOUR, GB [1 ]
COLQUHOUN, IJ [1 ]
DUPONT, MS [1 ]
PARSLEY, KR [1 ]
SELVENDRAN, RR [1 ]
机构
[1] INST FOOD RES, NORWICH LAB, COLNEY LANE, NORWICH NR4 7UA, NORFOLK, ENGLAND
关键词
!sup]13[!/sup]CNMR spectroscopy; cell wall polysaccharides; fruit ripening; Lycopersicon esculentum; pectic galactans; Solanaceae; tomato;
D O I
10.1016/0031-9422(90)80008-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cell wall material was isolated from the pericarp of unripe and ripe tomatoes, free from intracellular compounds and active wall degrading enzymes. The wall preparations were sequentially extracted with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA) at 20°, 0.05 M Na2CO3 at 1°, 0.05 M Na2CO3 at 20°, and 0.5, 1 and 4 M KOH at 20° to leave the α-cellulose residue, which contained a significant amount of pectic material. The polysaccharides isolated from the extracts were fractionated by anion-exchange chromatography and selected fractions were subjected to methylation analysis. The CDTA-soluble pectic polysaccharides had slightly-branched rhamnogalacturonan back-bones compared with the sodium carbonate-soluble pectic polysaccharides. The side chains of the pectic polysaccharides were mainly composed of β-(1→4)-Iinked galactopyranosyl and α-(1→5)-Iinked arabinofuranosyl residues, and the evidence for this was obtained by both methylation analysis and 13C NMR spectroscopy. The major hemicellulosic polysaccharide was a xyloglucomannan and there was evidence for the occurrence of a small amount of a xylan-pectic complex. The ripe fruit contained much less pectic galactans compared with the unripe fruit, and there was a significant decrease in the content of galactan side-chains of the ripe fruit. The hemicelluloses of the unripe and ripe fruit, however, showed negligible difference in composition. © 1990.
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收藏
页码:725 / 731
页数:7
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