REVERSAL BY EGTA OF THE ENHANCED SECRETORY RESPONSIVENESS OF MAST-CELLS DUE TO TREATMENT WITH OUABAIN

被引：12

作者：

JOHANSEN, T

KNUDSEN, T

BERTELSEN, H

机构：

[1] Department of Pharmacology, The Medical School, Odense University, DK-5000 Odense C

来源：

FEBS LETTERS | 1990年 / 262卷 / 02期

关键词：

ATPase; Na[!sup]+[!/sup; K[!sup]+[!/sup]-; Ca[!sup]2+[!/sup; Chelating agent; EGTA; Mast cell; Ouabain;

D O I：

10.1016/0014-5793(90)80196-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of EGTA on the enhancement by ouabain of compound 48 80-induced secretion from mast cells was compared with the effect on the Na+-K+ pump activity. The time-dependent secretory enhancement by ouabain was blocked by addition of EGTA to the cell suspension concomitantly with the addition of ouabain, and EGTA caused a large increase in the pump activity. Addition of 10 μM EGTA to ouabain-treated cells stopped but did not reverse the enhancement. The experiments show that the effect of ouabain was due to changes in a calcium pool utilized in compound 48 80-induced secretion following changes in the Na+,K+ pump activity. © 1990.

引用

页码：228 / 230

页数：3

共 15 条

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