RADIOIMMUNOASSAYS FOR THE VINCA ALKALOIDS, VINBLASTINE AND VINCRISTINE

被引：22

作者：

LANGONE, JJ ^{[1
]}

DONOFRIO, MR ^{[1
]}

VANVUNAKIS, H ^{[1
]}

机构：

[1] BRANDEIS UNIV,DEPT BIOCHEM,WALTHAM,MA 02154

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90208-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Radioimmunoassays for the chemotherapeutic alkaloids, vinblastine (VLB) and vincristine (VCR) were developed that could determine less than 1 pmol of each compound in the homologous hapten-antibody reaction. The anti-VCR serum bound VCR 200 times more effectively than VLB, demonstrating a high specificity in spite of the structural similarity of the drugs. Several other chemotherapeutic drugs showed no significant cross-reactivity. The assays were used to measure plasma levels of the alkaloids in rabbits treated with the drugs and to determine immunoreactive drug equivalents in extracts of the periwinkle plant following fractionation by high-pressure liquid chromatography. © 1979.

引用

页码：214 / 221

页数：8

共 27 条

[1] AROSHIMA M, 1973, CANCER, V34, P227
[2] CASE DC, 1977, CANCER-AM CANCER SOC, V39, P1382, DOI 10.1002/1097-0142(197704)39:4<1382::AID-CNCR2820390405>3.0.CO
[3] 2-F
[4] CASTLE MC, 1976, CANCER RES, V36, P3684
[5] INVESTIGATIONS OF METABOLIC-FATE OF TRITIATED VINCRISTINE IN RAT BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY
CASTLE, MC
MEAD, JAR
[J]. BIOCHEMICAL PHARMACOLOGY, 1978, 27 (01) : 37 - 44
[6] CREASEY WA, 1975, CANCER RES, V35, P1116
[7] 1,3-BIS(2-CHLOROETHYL)-1-NITROSOUREA(BCNU), CYCLOPHOSPHAMIDE, VINCRISTINE-(BCOP) AND PREDNISONE-(BCOP) - NEW THERAPEUTIC REGIMEN FOR DIFFUSE HISTIOCYTIC LYMPHOMA
DURANT, JR
LOEB, V
DORFMAN, R
CHAN, YK
[J]. CANCER, 1975, 36 (06) : 1936 - 1944
[8] GRENIUS HF, 1968, J MED CHEM, V11, P254
[9] JOHNSON IS, 1963, CANCER RES, V23, P1390
[10] ANTIBODIES - ANALYTICAL TOOLS TO STUDY PHARMACOLOGICALLY ACTIVE COMPOUNDS
LANGONE, JJ
VANVUNAKIS, H
LEVINE, L
[J]. ACCOUNTS OF CHEMICAL RESEARCH, 1975, 8 (10) : 335 - 342

← 1 2 3 →