GCMS-IDENTIFIED STEROIDS AND STEROID GLUCORONIDES IN GONADS AND HOLDING WATER OF TRICHOGASTER-TRICHOPTERUS (ANABANTIDAE, PALLAS 1770)

1. Analysis by gas chromatography-mass spectrometry was performed to identify steroids and steroid glucoronides in gonads of the tropical fish Trichogaster trichopterus and in the water in which the fish were maintained. 2. Full mass spectra of estradiol-17-beta (E2), testosterone, 17-alpha-hydroxyprogesterone, cholesterol, stigmasterol, 4-beta-methylcholesterol, estrone, 17-alpha,20-beta-hydroxy-4-pregnen-3-one (17,20-P) and sitosterol were obtained. 3. The above steroids were detected in both female and male gonads, with the exception of estrone, which was detected only in the male, and 17,20-P, which was detected only in the female. 4. All steroids except 17,20-P were detected in the water in which the fish were maintained.