EXPRESSION OF A BARLEY RIBOSOME-INACTIVATING PROTEIN LEADS TO INCREASED FUNGAL PROTECTION IN TRANSGENIC TOBACCO PLANTS

被引：167

作者：

LOGEMANN, J ^{[1
]}

JACH, G ^{[1
]}

TOMMERUP, H ^{[1
]}

MUNDY, J ^{[1
]}

SCHELL, J ^{[1
]}

机构：

[1] CARLSBERG RES LAB,DK-2500 VALBY,DENMARK

来源：

BIO-TECHNOLOGY | 1992年 / 10卷 / 03期

关键词：

D O I：

10.1038/nbt0392-305

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have obtained transgenic tobacco plants that express a barley ribosome inactivating protein (RIP) under control of the wound-inducible promoter of the potato wun1 gene. R0 transformants selected on kanamycin were tested for growth in soil inoculated with the soil-borne phytopathogen Rhizoctonia solani. Three R0 plants, which grew well compared to controls, and which were shown to carry the transgene by Southern blotting, were selfed and the R1 progeny tested for protection against fungal inoculation. Southern and 2-D Western blotting showed that R1 plants exhibiting increased protection had inherited the transgene and expressed the barley RIP upon wounding.

引用

页码：305 / 308

页数：4

共 26 条

[1] ISOLATION AND CHARACTERIZATION OF INHIBITORS OF ANIMAL CELL-FREE PROTEIN-SYNTHESIS FROM BARLEY-SEEDS
ASANO, K
SVENSSON, B
POULSEN, FM
[J]. CARLSBERG RESEARCH COMMUNICATIONS, 1984, 49 (07) : 619 - 626
[2] BINARY AGROBACTERIUM VECTORS FOR PLANT TRANSFORMATION
BEVAN, M
[J]. NUCLEIC ACIDS RESEARCH, 1984, 12 (22) : 8711 - 8721
[3] Boller T., 1988, Oxford Surveys of Plant Molecular and Cell Biology, V5, P145
[4] BROGLIE R, 1991, 3RD INT C INT SOC PL, pP1364
[5] INHIBITORS OF ANIMAL CELL-FREE PROTEIN-SYNTHESIS FROM GRAINS
COLEMAN, WH
ROBERTS, WK
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 696 (03) : 239 - 244
[6] DUNSMUIR P, 1991, 3RD INT C INT SOC PL, pP1313
[7] DUNSMUIR P, 1991, 3RD INT C INT SOC PL, pP1364
[8] ORGAN-SPECIFIC EXPRESSION OF 3 LEAF STEM SPECIFIC CDNAS FROM POTATO IS REGULATED BY LIGHT AND CORRELATED WITH CHLOROPLAST DEVELOPMENT
ECKES, P
SCHELL, J
WILLMITZER, L
[J]. MOLECULAR & GENERAL GENETICS, 1985, 199 (02): : 216 - 224
[9] THE MECHANISM OF ACTION OF BARLEY TOXIN - A TYPE-1 RIBOSOME-INACTIVATING PROTEIN WITH RNA N-GLYCOSIDASE ACTIVITY
ENDO, Y
TSURUGI, K
EBERT, RF
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 954 (02) : 224 - 226
[10] JACH G, 1992, BIOPRACTICE, V1, P1

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