HISTAMINE, ACTIN-GELSOLIN BINDING, AND POLYPHOSPHOINOSITIDES IN HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS

被引:23
作者
CARSON, MR
SHASBY, SS
LIND, SE
SHASBY, DM
机构
[1] UNIV IOWA,COLL MED,DEPT INTERNAL MED,IOWA CITY,IA 52242
[2] VET AFFAIRS HOSP,IOWA CITY,IA 52242
[3] HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DEPT MED,DIV HEMATOL ONCOL,BOSTON,MA 02115
[4] HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DEPT MED,DIV EXPTL MED,BOSTON,MA 02115
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1992年 / 263卷 / 06期
关键词
HISTAMINE; ENDOTHELIUM;
D O I
10.1152/ajplung.1992.263.6.L664
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Histamine activates inositol phospholipid metabolism, increases calcium, and causes a change in shape of human umbilical vein endothelial (HUVE) cells. Changes in endothelial cell shape are determined, in part, by changes in the actin cytoskeleton. Gelsolin is an actin-binding protein with the potential to alter the actin cytoskeleton in response to changes in cell calcium and/or changes in polyphosphoinositides. Therefore, we examined the interactions of actin and gelsolin in HUVE cells in which inositol phospholipid metabolism was activated with histamine. In HUVE cells exposed to histamine we estimated actin-gelsolin binding by quantitating actin and gelsolin, immunoprecipitated with anti-gelsolin Sepharose. We estimated the relative amount of filamentous actin in the histamine-exposed HUVE cells by quantitating the amount of actin that was Triton soluble. We also measured the amount of phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2) in the HUVE cells before and after exposure to histamine. We found that histamine decreased the amount of actin that was immunoprecipitated with gelsolin, decreased the fraction of cell actin that was Triton soluble, and increased PIP and PIP2. These results demonstrate that histamine promotes actin filament formation in HUVE cells and that histamine-mediated changes in actin-gelsolin binding in these cells are better predicted by changes in polyphosphoinositides than by increases in cell calcium.
引用
收藏
页码:L664 / L669
页数:6
相关论文
共 35 条
[1]   QUANTIFICATION OF DAMAGE BY AIR EMBOLI TO LUNG MICROVESSELS IN ANESTHETIZED SHEEP [J].
ALBERTINE, KH ;
WIENERKRONISH, JP ;
KOIKE, K ;
STAUB, NC .
JOURNAL OF APPLIED PHYSIOLOGY, 1984, 57 (05) :1360-1368
[2]  
BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911
[3]  
CARSON MR, 1989, AM J PHYSIOL, V257, pL264
[4]   REVERSIBILITY OF GELSOLIN/ACTIN INTERACTION IN MACROPHAGES - EVIDENCE OF CA-2+-DEPENDENT AND CA-2+-INDEPENDENT PATHWAYS [J].
CHAPONNIER, C ;
YIN, HL ;
STOSSEL, TP .
JOURNAL OF EXPERIMENTAL MEDICINE, 1987, 165 (01) :97-106
[5]   THE ACTIN FILAMENT SEVERING DOMAIN OF PLASMA GELSOLIN [J].
CHAPONNIER, C ;
JANMEY, PA ;
YIN, HL .
JOURNAL OF CELL BIOLOGY, 1986, 103 (04) :1473-1481
[6]   ENHANCED MOTILITY IN NIH-3T3 FIBROBLASTS THAT OVEREXPRESS GELSOLIN [J].
CUNNINGHAM, CC ;
STOSSEL, TP ;
KWIATKOWSKI, DJ .
SCIENCE, 1991, 251 (4998) :1233-1236
[7]   LACK OF CORRELATION BETWEEN CHANGES IN POLYPHOSPHOINOSITIDE LEVELS AND ACTIN GELSOLIN COMPLEXES IN A431 CELLS TREATED WITH EPIDERMAL GROWTH-FACTOR [J].
DADABAY, CY ;
PATTON, E ;
COOPER, JA ;
PIKE, LJ .
JOURNAL OF CELL BIOLOGY, 1991, 112 (06) :1151-1156
[8]  
EBERLE M, 1990, J BIOL CHEM, V265, P16725
[9]   ASSOCIATION OF GELSOLIN WITH ACTIN-FILAMENTS AND CELL-MEMBRANES OF MACROPHAGES AND PLATELETS [J].
HARTWIG, JH ;
CHAMBERS, KA ;
STOSSEL, TP .
JOURNAL OF CELL BIOLOGY, 1989, 108 (02) :467-479
[10]   COTRANSLATIONAL ASSEMBLY OF MYOSIN HEAVY-CHAIN IN DEVELOPING CULTURED SKELETAL-MUSCLE [J].
ISAACS, WB ;
FULTON, AB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (17) :6174-6178