A DUAL DOMINANT SELECTION, DUAL MARKER GENE AMPLIFICATION MODEL FOR ENVIRONMENTAL SURVEILLANCE OF RECOMBINANT VIRAL VACCINES

被引：4

作者：

KATZ, JB

机构：

来源：

RESEARCH IN VIROLOGY | 1990年 / 141卷 / 06期

关键词：

DNA; PCR; POXVIRUS; HERPESVIRUS; RECOMBINANT VACCINE; GENE MODEL; ENVIRONMENTAL SURVEILLANCE;

D O I：

10.1016/0923-2516(90)90032-E

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A simple dual dominant selection marker gene model is proposed for recombinant DNA (rDNA) viral vaccines. Marker genes are also used as targets for in vitro DNA amplification (polymerase chain reaction). Environmentally stable and destabilized rDNA viruses are easily recovered and identified. Use of the model would facilitate evaluation of pressing environmental and safety issues surrounding release of rDNA herpesviruses and poxviruses into the biosphere.

引用

页码：591 / 596

页数：6

共 31 条

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