CHARACTERIZATION OF IMMUNE-COMPLEXES BY ISOELECTRIC-FOCUSING IN AGAROSE GELS

被引：6

作者：

HOFFMAN, WL ^{[1
]}

KELLY, PJ ^{[1
]}

LARRUMBIDE, M ^{[1
]}

机构：

[1] UNIV TEXAS,SW MED CTR,DEPT INTERNAL MED,DALLAS,TX 75235

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 132卷 / 01期

关键词：

(Antibody); (Antigen); Agarose gel; Immune complex; Isoelectric focusing; Urea;

D O I：

10.1016/0022-1759(90)90403-I

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for the characterization of immune complexes on thin-layer agarose isoelectric focusing (IEF) gels. This method involves dissociating immune complexes and then maintaining this dissociation during IEF in agarose gels containing 9 M urea. After IEF, the immune complex components can be quantitatively transferred to nitrocellulose in less than 15 min, and a variety of immunostaining procedures can be used to probe these blotted components. No loss of biological activity was detected in any of the blotted components. © 1990.

引用

页码：103 / 110

页数：8

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