A CLONED ANTIGEN FOR SEROLOGICAL DIAGNOSIS OF WUCHERERIA-BANCROFTI MICROFILAREMIA WITH DAYTIME BLOOD-SAMPLES

被引：42

作者：

DISSANAYAKE, S

XU, M

PIESSENS, WF

机构：

[1] Harvard School of Public Health, Boston, MA

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1992年 / 56卷 / 02期

关键词：

WUCHERERIA-BANCROFTI; MICROFILAREMIA; ELISA; SERODIAGNOSIS; RECOMBINANT ANTIGEN; FILARIASIS;

D O I：

10.1016/0166-6851(92)90176-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By differentially screening an adult Brugia malayi cDNA library with sera from microfilaremic and amicrofilaremic donors infected with Wuchereria bancrofti, we have identified a novel parasite antigen denoted SXP-1. Recombinant SXP-1 filarial antigen is preferentially recognized by sera from microfilaremic persons with bancroftian filariasis and from skin snip-positive patients with onchocerciasis. Antibodies to SXP-1 are restricted to the IgG4 subclass and gradually decline after treatment with diethylcarbamazine. These findings indicate that it may be possible to replace microscopic examination of night blood films with a serological test designed to detect antibodies to a mix of SXP-1 and other suitable antigens for the diagnosis of microfilaremia due to bancroftian filariasis.

引用

页码：269 / 277

页数：9

共 20 条

[1]

ALMOND NM, 1986, IMMUNOLOGY, V59, P633

[2]

DENNIS DT, 1970, AM J TROP MED HYG, V25, P257

[3]

DISSANAYAKE S, 1987, CIBA F SYMP, V127, P203

[4] MICROFILARAEMIA, SERUM ANTIBODY AND DEVELOPMENT OF CLINICAL-DISEASE IN MICROFILAREMIC SUBJECTS INFECTED WITH WUCHERERIA-BANCROFTI AND TREATED WITH DIETHYLCARBAMAZINE CITRATE [J].