IDENTITY OF AMINO-ACID-SEQUENCES OF SUPEROXIDE-DISMUTASE PURIFIED FROM BOTH ANAEROBICALLY MAINTAINED AND AERATED PORPHYROMONAS-GINGIVALIS

被引：9

作者：

AMANO, A ^{[1
]}

SHIZUKUISHI, S ^{[1
]}

TSUNEMITSU, A ^{[1
]}

TSUNASAWA, S ^{[1
]}

机构：

[1] OSAKA UNIV,FAC DENT,DEPT PREVENT DENT,1-8 YAMADAOKA,SUITA,OSAKA 565,JAPAN

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1992年 / 7卷 / 06期

关键词：

PORPHYROMONAS-GINGIVALIS; SUPEROXIDE DISMUTASE; AMINO ACID SEQUENCE;

D O I：

10.1111/j.1399-302X.1992.tb00638.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Superoxide dismutase (SOD) from anaerobically maintained Porphyromonas gingivalis (anaero-SOD) has the characteristic of Fe-SOD, whereas SOD from aerated cells (aero-SOD) has that of Mn-SOD. However, both types of apoSOD can bind either Fe or Mn. To elucidate the structure relationship between anaero- and aero-SOD, we examined the amino acid sequence of aero-SOD by direct protein analysis. The amino acid sequence of aero-SOD was shown to be identical with that of anaero-SOD determined previously. Our findings support the hypothesis that cambialistic SOD is formed from a single apoprotein in bacterial cells.

引用

页码：368 / 371

页数：4

共 11 条

[1]

Amano A, Shizukuishi S, Tamagawa H, Iwakura K, Tsunasawa H, Tsunemitsu A., Characterization of superoxide dis‐mutase purified from either anaerobically maintained or aerated Bacteroides gingivalis, J Bacteriol, 172, pp. 1457-1463, (1990)

[2]

Amano A, Shizukuishi S, Tsunemitsu A, Maekawa K, Tsunasawa S., The primary structure of superoxide dismutase purified from anaerobically maintained Bacteroides gingivalis, FEBS Lett, 272, pp. 217-220, (1990)

[3]

Gregory EM, Dapper CH, Isolation of iron‐containing superoxide dismutase from Bacteroides fragilis: reconstitution as a Mn‐containing enzyme, Arch Bio-chem Biophys, 220, pp. 293-300, (1983)

[4]

Gregory EM, Characterization of the O<sup>2</sup>‐induced manganese‐containing superoxide dismutase from Bacteroides fragilis, Arch Biochem Biophys, 238, pp. 83-89, (1985)

[5]

Hassan HM, Microbial superoxide dismutase, Adv Genet, 26, pp. 65-97, (1989)

[6]

Kirby T, Blum J, Kahane I, Fridovich I., Distinguishing between Mn‐containing and Fe‐containing superoxide dismutase in crude extracts of cells, Arch Biochem Biophys, 201, pp. 551-555, (1980)

[7]

Martin ME, Byers BR, Olson MOJ, Sal-in ML, Arceneaux JEL, Tolbert C., A Streptococcus mutans superoxide dismutase that is active with either manganese iron as a cofactor, J Biol Chem, 261, pp. 9361-9367, (1986)

[8]

Meier B, Barra D, Bossa IF, Caiabrese L, Rotilio G., Synthesis of either Fe‐ or Mn‐superoxide dismutase with an apparently identical protein moiety by an anaerobic bacterium dependent on the metal supplied, J Biol Chem, 257, pp. 13977-13980, (1982)

[9]

Parker MW, Blake CCF, Iron‐ and manganese‐containing superoxide dismutase can be distinguished by analysis of their primary structures, FEBS Lett, 229, pp. 377-382, (1988)

[10]

Pennington CD, Gregory EM, Isolation and reconstitution of iron‐ and manganese‐containing superoxide dismutase from Bacteroides thetaiotaomicron, J Bacteriol, 166, pp. 528-532, (1986)

← 1 2 →