DIPHENYLTIN DICHLORIDE AS A CHROMOGENIC REAGENT FOR THE DETECTION OF FLAVONOIDS ON THIN-LAYER PLATES

被引：4

作者：

HIERMANN, A

BUCAR, F

机构：

[1] Institute of Pharmacognosy, University of Graz, A-8010 Graz

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 675卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)85286-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Among several complexation reagents, diphenyltin dichloride (DTC) proved to be a useful chromogenic compound for both the qualitative and quantitative analysis of flavones and flavonols on thin-layer plates by forming fluorescent complexes of different colour. For qualitative analysis, comparative detection with DTC and diphenylboric acid 2-aminoethyl ester can indicate the position of the glycosidated hydroxyl of kaempferol and luteolin glycosides. Fluorimetric densitometry showed high sensitivity to flavonols with a free 5-hydroxyl group and flavones containing two adjacent hydroxyl groups in ring B.

引用

页码：276 / 281

页数：6

共 11 条

[1]

BILLETER M, 1990, Journal of Planar Chromatography - Modern TLC, V3, P370

[2]

GEISSMANN TA, 1962, CHEM FLAVONOID COMPO

[3]

HAIS IM, 1958, HDB PAPIERCHROMATOGR

[4]

Harborne J.B., 1975, FLAVONOIDS

[5]

HAYASHI T, 1970, CHEM PHARM BULL, V18, P1112

[6]

FLUORESCENCE AND STRUCTURE OF FLAVONES [J].

HOMBERG, H ;

GEIGER, H .

PHYTOCHEMISTRY, 1980, 19 (11) :2443-2449

[7]

JORK H, 1993, DUNNSCHICHTCHROMAT B, V1

[8]

JORK H, 1989, DUNNSCHICHTCHROMAT A, V1

[9]

AROMATISCHE BORSAUREN ALS BATHOCHROME REAGENTIEN FUR FLAVONE - ANALYTISCHE UNTERSUCHUNGEN AUF DEM FLAVON-GEBIET .8. [J].

NEU, R .

FRESENIUS ZEITSCHRIFT FUR ANALYTISCHE CHEMIE, 1956, 151 (05) :328-332

[10]

*EINE NEUE ORGANISCHE BORVERBINDUNG ALS ANALYTISCHES REAGENS [J].

NEU, R .

FRESENIUS ZEITSCHRIFT FUR ANALYTISCHE CHEMIE, 1954, 142 (05) :335-341

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