VERSATILE TRANSFORMATION VECTORS TO ASSAY THE PROMOTER ACTIVITY OF DNA ELEMENTS IN PLANTS

被引：38

作者：

PASQUALI, G ^{[1
]}

OUWERKERK, PBF ^{[1
]}

MEMELINK, J ^{[1
]}

机构：

[1] LEIDEN UNIV,INST MOLEC PLANT SCI,CLUSIUS LAB,2333 AL LEIDEN,NETHERLANDS

来源：

GENE | 1994年 / 149卷 / 02期

关键词：

AGROBACTERIUM TUMEFACIENS; GUSA; T-DNA; GENE EXPRESSION; CAMV; BINARY VECTORS;

D O I：

10.1016/0378-1119(94)90179-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A convenient vector system was developed to evaluate transcriptional promoter activities in plants. Two primary vectors, optionally containing the cauliflower mosaic virus (CaMV) 35S -47 or -90 minimal promoters, offer multiple sites for cloning the sequence of interest upstream from the beta-glucuronidase gene (gusA). The promoter-gusA cassette can be transferred to a binary vector containing the selectable neomycin phosphotransferase II-encoding gene (nptII) next to the left border. In addition, the transferred DNA (T-DNA) contains the chloramphenicol acetyltransferase gene (cat) driven by the CaMV 35S promoter. Activity of cat can serve as a reference for gusA expression to correct for effects of chromosomal position or T-DNA copy number.

引用

页码：373 / 374

页数：2

共 8 条

[1] NEW PLANT BINARY VECTORS WITH SELECTABLE MARKERS LOCATED PROXIMAL TO THE LEFT T-DNA BORDER [J].

BECKER, D ;

KEMPER, E ;

SCHELL, J ;

MASTERSON, R .

PLANT MOLECULAR BIOLOGY, 1992, 20 (06) :1195-1197

[2] REGULATED GENES IN TRANSGENIC PLANTS [J].

BENFEY, PN ;

CHUA, NH .

SCIENCE, 1989, 244 (4901) :174-181

[3] BINARY AGROBACTERIUM VECTORS FOR PLANT TRANSFORMATION [J].

BEVAN, M .

NUCLEIC ACIDS RESEARCH, 1984, 12 (22) :8711-8721

[4]

FANG RX, 1989, PLANT CELL, V1, P141, DOI 10.1105/tpc.1.1.141

[5]

FLUHR R, 1986, EMBO J, V5, P2065

[6] A METAL-DEPENDENT DNA-BINDING PROTEIN INTERACTS WITH A CONSTITUTIVE ELEMENT OF A LIGHT-RESPONSIVE PROMOTER [J].

LAM, E ;

KANOMURAKAMI, Y ;

GILMARTIN, P ;

NINER, B ;

CHUA, NH .

PLANT CELL, 1990, 2 (09) :857-866

[7]

SIJMONS PC, 1990, BIO-TECHNOL, V8, P217, DOI 10.1038/nbt0390-217

[8] RIGHT 25-BP TERMINUS SEQUENCE OF THE NOPALINE T-DNA IS ESSENTIAL FOR AND DETERMINES DIRECTION OF DNA TRANSFER FROM AGROBACTERIUM TO THE PLANT GENOME [J].

WANG, K ;

HERRERAESTRELLA, L ;

VANMONTAGU, M ;

ZAMBRYSKI, P .

CELL, 1984, 38 (02) :455-462

← 1 →