THE INFLUENCE OF METHYLPREDNISOLONE ON THE ENERGY-METABOLISM OF EHRLICH ASCITES TUMOR-CELLS

被引：7

作者：

BUTTGEREIT, F ^{[1
]}

MULLER, M ^{[1
]}

WOLBART, K ^{[1
]}

THIELE, B ^{[1
]}

HIEPE, F ^{[1
]}

机构：

[1] HUMBOLDT UNIV BERLIN,INST BIOCHEM,D-10115 BERLIN,GERMANY

来源：

BIOSCIENCE REPORTS | 1994年 / 14卷 / 06期

关键词：

EHRLICH ASCITES TUMOR CELLS; ENERGY METABOLISM; METHYLPREDNISOLONE; CELL MEMBRANE; PHOSPHOLIPID TURNOVER; CALCIUM;

D O I：

10.1007/BF01199052

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using Ehrlich ascites tumour cells, the short-term effects of the therapeutic glucocorticoid Methylprednisolone (MP) on the cellular energy metabolism were studied. ATP-consuming processes involved in the rapid MP effects were identified indirectly from the effects of MP on cellular oxygen consumption related to the inhibition of respiration by selective inhibitors of Ca2+-ATPase and protein synthesis. The effects of MP on plasma membrane permeability for Ca2+ ions and phospholipid turnover were studied directly by using confocal laser scanning microscopy and tracerkinetic measurements, respectively. MP inhibited cellular oxygen consumption, suppressed the inhibitory effect of lanthanum but not that of cycloheximide on oxygen consumption, blocked the [Ca2+](i) rise in response to calcium ionophore A 23187, and decreased phospholipid turnover. MP acted instantly in a dose-dependent manner. The observed effects of MP are discussed in relation to the hypothesis that the drug has direct membrane effect affecting plasma membrane permeability and function.

引用

页码：283 / 290

页数：8

共 28 条

[1] EVIDENCE FOR COLOCALIZATION OF GLUCOCORTICOID RECEPTOR WITH CYTOPLASMIC MICROTUBULES IN HUMAN GINGIVAL FIBROBLASTS, USING 2 DIFFERENT MONOCLONAL ANTI-GR ANTIBODIES, CONFOCAL LASER SCANNING MICROSCOPY AND IMAGE-ANALYSIS [J].

AKNER, G ;

MOSSBERG, K ;

WIKSTROM, AC ;

SUNDQVIST, KG ;

GUSTAFSSON, JA .

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1991, 39 (04) :419-432

[2] MEGADOSE METHYLPREDNISOLONE PULSE THERAPY IN ADULT IDIOPATHIC THROMBOCYTOPENIC PURPURA [J].

AKOGLU, T ;

PAYDAS, S ;

BAYIK, M ;

LAWRENCE, R ;

FIRATLI, T .

LANCET, 1991, 337 (8732) :56-56

[3]

Anderson D K, 1985, Cent Nerv Syst Trauma, V2, P257

[4]

ARDAWI MSM, 1908, ESSAYS BIOCHEM, V21, P1

[5]

ARSLAN P, 1985, J BIOL CHEM, V260, P2719

[6]

BARILE L, 1992, J RHEUMATOL, V19, P370

[7] EFFECT OF ANTIRHEUMATIC DRUGS ON LYMPHOCYTE MEMBRANE FLUIDITY IN RHEUMATOID-ARTHRITIS - A FLUORESCENCE POLARIZATION STUDY [J].

BECCERICA, E ;

PIERGIACOMI, G ;

CURATOLA, G ;

FERRETTI, G .

PHARMACOLOGY, 1989, 38 (01) :16-22

[8] METHYLPREDNISOLONE OR NALOXONE TREATMENT AFTER ACUTE SPINAL-CORD INJURY - 1-YEAR FOLLOW-UP DATA - RESULTS OF THE 2ND NATIONAL ACUTE SPINAL-CORD INJURY STUDY [J].

BRACKEN, MB ;

SHEPARD, MJ ;

COLLINS, WF ;

HOLFORD, TR ;

BASKIN, DS ;

EISENBERG, HM ;

FLAMM, E ;

LEOSUMMERS, L ;

MAROON, JC ;

MARSHALL, LF ;

PEROT, PL ;

PIEPMEIER, J ;

SONNTAG, VKH ;

WAGNER, FC ;

WILBERGER, JL ;

WINN, HR ;

YOUNG, W .

JOURNAL OF NEUROSURGERY, 1992, 76 (01) :23-31

[9] EFFECTS OF METHYLPREDNISOLONE ON THE ENERGY-METABOLISM OF QUIESCENT AND CONA-STIMULATED THYMOCYTES OF THE RAT [J].

BUTTGEREIT, F ;

BRAND, MD ;

MULLER, M .

BIOSCIENCE REPORTS, 1993, 13 (01) :41-52

[10]

BUTTGEREIT F, 1991, BIOCHEM INT, V24, P59

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