GENE-REGULATION BY TEMPERATURE-SENSITIVE P53 MUTANTS - IDENTIFICATION OF P53 RESPONSE GENES

被引：81

作者：

BUCKBINDER, L ^{[1
]}

TALBOTT, R ^{[1
]}

SEIZINGER, BR ^{[1
]}

KLEY, N ^{[1
]}

机构：

[1] BRISTOL MYERS SQUIBB PHARMACEUT RES INST,DEPT MOLEC GENET & CELL BIOL,PRINCETON,NJ 08540

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 22期

关键词：

D O I：

10.1073/pnas.91.22.10640

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

The ability of the p53 protein to act as a sequence-specific transcriptional activator suggests that genes induced by p53 may encode critical mediators of p53 tumor suppression. Using a tetracycline-regulated p53 expression system and cDNA library subtraction procedure, we identified several p53-induced gene transcripts in human Saos-2 osteosarcoma cells that are novel on the basis of their size, regulation, and low abundance. Wild type p53-dependent induction of these transcripts was observed in cells that are growth arrested by p53, as well as in cells that undergo apoptosis upon expression of an inducible wild-type p53 transgene. These results show that p53 activates the expression of numerous response genes and suggest that multiple effecters may play a role in mediating cellular functions of p53.

引用

页码：10640 / 10644

页数：5

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