CLONING AND SEQUENCING OF THE GENES ENCODING ACID-SOLUBLE SPORE PROTEINS FROM CLOSTRIDIUM-PERFRINGENS

被引：8

作者：

HOLCK, A ^{[1
]}

BLOM, H ^{[1
]}

GRANUM, PE ^{[1
]}

机构：

[1] NORWEGIAN COLL VET MED,DEPT FOOD HYG,OSLO,NORWAY

来源：

GENE | 1990年 / 91卷 / 01期

关键词：

genomic fragment; oligodeoxyribonucleotide probe; phage; λ; library; plasmid vector; Recombinant DNA; spore germination; storage protein;

D O I：

10.1016/0378-1119(90)90169-R

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

In Clostridium perfringens the acid-soluble spore proteins (ASSPs) of the α/ß type are encoded by two genes, sspC1 and sspC2. These genes, encoding ASSP C1 and C2 proteins, respectively, were cloned as parts of 3.5-kb genomic fragments. The genes were sequenced and polypeptides of 59 (C1) and 60 (C2) amino acids (aa) were deduced from the nucleotide sequence. The N-terminal Met is removed from the primary translation products in both cases, yielding mature polypeptides with Mrs of 6306 (C1) and 6553 (C2). The aa sequences of the two polypeptides were very similar and showed a closer relationship with C. bifermentans ASSPs than with ASSPs from Bacillus species. © 1990.

引用

页码：107 / 111

页数：5

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[2]

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[3]

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