THE 2-HYBRID SYSTEM - AN ASSAY FOR PROTEIN-PROTEIN INTERACTIONS

被引：491

作者：

FIELDS, S ^{[1
]}

STERNGLANZ, R ^{[1
]}

机构：

[1] SUNY STONY BROOK,DEPT BIOCHEM & CELL BIOL,STONY BROOK,NY 11794

来源：

TRENDS IN GENETICS | 1994年 / 10卷 / 08期

关键词：

D O I：

10.1016/0168-9525(90)90012-U

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The two-hybrid system is a yeast-based genetic assay for detecting protein-protein interactions. It can be used to identify proteins that bind to a protein of interest, or to delineate domains or residues critical for an interaction. Variations on this methodology have been developed to clone genes that encode DNA-binding proteins, to identify, peptides that bind to a protein and, potentially, to screen for drugs.

引用

页码：286 / 292

页数：7

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