CHARACTERIZATION OF AZOSPIRILLUM AND RELATED DIAZOTROPHS ASSOCIATED WITH ROOTS OF PLANTS GROWING IN SALINE SOILS

被引：25

作者：

BILAL, R ^{[1
]}

RASUL, G ^{[1
]}

QURESHI, JA ^{[1
]}

MALIK, KA ^{[1
]}

机构：

[1] NUCL INST AGR & BIOL,POB 128,JHANG RD,FAISALABAD,PAKISTAN

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1990年 / 6卷 / 01期

关键词：

D O I：

10.1007/BF01225354

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Diazotrophs, especially of genus Azospirillum were readily isolated from roots of many plants using semi-solid nitrogen free malate medium (NFM). These isolates formed fine, white sub-surface pellicle in nitrogen-free malate medium within 24h, which gradually moved to the surface, and exhibited high acetylene reduction rates. Using selected cultural and biochemical tests, most of the isolates were identified as Azospirillum brasilense. Four isolates from Kallar grass root surface and one isolate from Atriplex root interior formed phenotypically a homogenous group. It shared many characteristics with the species of genus Azospirillum except shape. All the biochemical tests performed, categorized them with A. brasilense. However, the shape and the protein profile on SDS polyacrylamide gel electrophoretograms suggested that the group of these five isolates is clearly distinct and differs widely from all the type strains, belonging to various genera. © 1990 Rapid Communications of Oxford Ltd.

引用

页码：46 / 52

页数：7

共 20 条

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