ISOLATION AND CHARACTERIZATION OF BACILLUS-SUBTILIS MUTANTS BLOCKED IN THE SYNTHESIS OF PANTOTHENIC-ACID

被引:11
作者
BAIGORI, M [1 ]
GRAU, R [1 ]
MORBIDONI, HR [1 ]
DEMENDOZA, D [1 ]
机构
[1] UNIV NACL SUR,FAC CIENCIAS BIOQUIM & FARMACEUT,DEPT MICROBIOL,RA-8000 BAHIA BLANCA,ARGENTINA
关键词
D O I
10.1128/jb.173.13.4240-4242.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have produced and characterized by physiological and enzymatic analyses pantothenate (pan) auxotrophs of Bacillus subtilis. panB auxotrophs are deficient in ketopantoate hydroxymethyltransferase, whereas panE mutants lack ketopantoic acid reductase. The pan mutations were mapped by phage PBS1-mediated two-factor crosses and found to be located in the interval purE-tre of the genetic map of B. subtilis.
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页码:4240 / 4242
页数:3
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