DETECTION OF A CPA METHYLASE IN AN INSECT SYSTEM - CHARACTERIZATION AND SUBSTRATE-SPECIFICITY

被引：12

作者：

DEVAJYOTHI, C

BRAHMACHARI, V

机构：

[1] INDIAN INST SCI, DEPT MICROBIOL & CELL BIOL, BANGALORE 560012, KARNATAKA, INDIA

[2] INDIAN INST SCI, ICMR CTR GENET & CELL BIOL, BANGALORE 560012, KARNATAKA, INDIA

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 1992年 / 110卷 / 02期

关键词：

DNA METHYLATION; COCCIDS; PROCESSIVE ENZYME; RNA INHIBITION; CPI METHYLATION;

D O I：

10.1007/BF02454187

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A cytosine-specific DNA methyltransferase (EC 2.1.1.37) has been purified to near homogeneity from a mealybug (Planococcus lilacinus). The enzyme can methylate cytosine residues in CpG sequences as well as CpA sequences. The apparent molecular weight of the enzyme was estimated as 135,000 daltons by FPLC. The enzyme exhibits a processive mode of action and a salt dependance similar to mammalian methylases. Mealybug methylase exhibits a preference for denatured DNA substrates.

引用

页码：103 / 111

页数：9

共 34 条

[1] A SENSITIVE IMMUNOCHEMICAL METHOD FOR DETECTING 5MC IN DNA FRAGMENTS [J].