IDENTIFICATION OF CAMPYLOBACTER-FETUS BY PCR-DNA PROBE METHOD

被引：20

作者：

BLOM, K ^{[1
]}

PATTON, CM ^{[1
]}

NICHOLSON, MA ^{[1
]}

SWAMINATHAN, B ^{[1
]}

机构：

[1] CTR DIS CONTROL & PREVENT,NATL CTR INFECT DIS,DIV BACTERIAL & MYCOT DIS,ATLANTA,GA 30333

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 05期

关键词：

D O I：

10.1128/JCM.33.5.1360-1362.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A PCR method for rapid identification of Campylobacter fetus subsp. fetus was evaluated. A fragment of the gene coding for 16S rRNA was amplified from crude cell lysates of 18 C. fetus strains and 30 strains representing other Campylobacter species and subspecies. The amplicons were probed by dot blot hybridization with a digoxigenin-labeled C. fetus-specific oligonucleotide probe. The probe reacted only with C. fetus subsp. fetus and C. fetus subsp. venerealis and may be useful for rapid identification in clinical laboratories.

引用

页码：1360 / 1362

页数：3

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