SEPARATION AND CHARACTERIZATION OF HIGH-DENSITY-LIPOPROTEIN SUBPOPULATIONS BY GEL-PERMEATION CHROMATOGRAPHY

被引：23

作者：

CLIFTON, PM ^{[1
]}

MACKINNON, AM ^{[1
]}

BARTER, PJ ^{[1
]}

机构：

[1] FLINDERS UNIV S AUSTRALIA, MED CTR, DEPT BIOCHEM, BEDFORD PK, SA 5042, AUSTRALIA

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1987年 / 414卷 / 01期

关键词：

D O I：

10.1016/0378-4347(87)80021-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

High density lipoproteins (HDL) contain at least five distinct subpopulations when analyzed by gradient gel electrophoresis. This report represents the first description of a simple technique for isolating these subpopulations of HDL in quantities sufficient to enable characterization in terms of particle size, apolipoprotein AII content and chemical composition. Lipoproteins were separated and subfractionated on a column of Superose 6B using a fast protein liquid chromatography system. Five normal subjects were studied: HDL2b and HDL3a were isolated as essentially single subpopulations from all subjects, while HDL2a could be isolated from only three of the subjects. HDL3b was isolated in a relatively impure form (70%) from all subjects. Identical subpopulations were identified in each subject by gradient gel electrophoresis of unseparated HDL.

引用

页码：25 / 34

页数：10

共 19 条

[1] PRECIPITATION OF I-125 LABELED LIPOPROTEINS WITH SPECIFIC POLYPEPTIDE ANTISERA - EVIDENCE FOR 2 POPULATIONS WITH DIFFERING POLYPEPTIDE COMPOSITIONS IN HUMAN HIGH DENSITY LIPOPROTEINS [J].

ALBERS, JJ ;

ALADJEM, F .

BIOCHEMISTRY, 1971, 10 (18) :3436-&

[2] PARTICLE DISTRIBUTION OF HUMAN-SERUM HIGH-DENSITY LIPOPROTEINS [J].

ANDERSON, DW ;

NICHOLS, AV ;

FORTE, TM ;

LINDGREN, FT .

BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 493 (01) :55-68

[3] CHARACTERIZATION OF HUMAN HIGH-DENSITY LIPOPROTEINS BY GRADIENT GEL-ELECTROPHORESIS [J].

BLANCHE, PJ ;

GONG, EL ;

FORTE, TM ;

NICHOLS, AV .

BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 665 (03) :408-419

[4]

BORRESEN AL, 1980, ARTERY, V7, P139

[5]

CHEUNG MC, 1979, J LIPID RES, V20, P200

[6]

CHEUNG MC, 1984, J BIOL CHEM, V259, P2201

[7]

DELALLA OF, 1954, METHOD BIOCHEM ANAL, V1, P459

[8] RAPID SEPARATION OF PLASMA-LIPOPROTEINS BY GEL-PERMEATION CHROMATOGRAPHY ON AGAROSE-GEL SUPEROSE-6B [J].

HA, YC ;

BARTER, PJ .

JOURNAL OF CHROMATOGRAPHY, 1985, 341 (01) :154-159

[9] SUBFRACTIONATION AND CHARACTERIZATION OF NATIVE AND INCUBATION ENLARGED HUMAN-PLASMA HIGH-DENSITY LIPOPROTEIN PARTICLES BY HIGH-PERFORMANCE GEL-FILTRATION [J].

HOLMQUIST, L ;

CARLSON, LA .

LIPIDS, 1985, 20 (06) :378-392

[10] STUDIES ON 2 ALPHA-LIPOPROTEIN BANDS THAT APPEAR IN AGAROSE GELELECTROPHORESIS OF HUMAN SERUM [J].

KOSTNER, G ;

DEPISCH, A ;

PETEK, W ;

HOLASEK, A .

HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1971, 352 (10) :1440-&

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