GERM-CELL REGULATION OF SERTOLI-CELL TRANSFERRIN MESSENGER-RNA LEVELS

Sertoli cells ct ltured in the presence of germ cells responded by increasing the level of transferrin mRNA 3-fold as determined by solution hybridization and Northern blot analysis. In contrast, the steady state levels of other mRNAs, including sulfated glycoprotein 1 (SGP-1), sulfated glycoprotein 2 (SGP-2), transferrin receptor, regulatory subunit of cAMP dependent protein kinase, and ferritin light chain, were not influenced by coculture with germ cells. The transferrin mRNA stimulatory activity was found in conditioned medium from germ cells but was not associated with germ cell membrane components. The activity was abolished by treatment of the medium with trypsin. Partial characterization and isolation of the protein(s) from conditioned medium indicated that it has an apparent mol wt between 10 and 30 K. Studies using inhibitors of protein and nucleic acid synthesis indicated that the stimulation of transferrin mRNA by germ cell conditioned medium required both transcription and translation. Sertoli cell enriched (germ cell depleted) testes were obtained from male offspring of pregnant females irradiated at the 19th day of gestation. Testicular transferrin mRNA levels from irradiated rats decreased in comparison to levels in the normal rat, whereas SGP-2 mRNA levels were unchanged. These studies demonstrate that germ cell secretions may interact with Sertoli cells to specifically increase the level of transferrin mRNA and that this interaction may be a mechanism by which germ cells regulate the flow of iron across the seminiferous epithelium. © 1990 by The Endocrine Society.