EFFECT OF ESTROGEN ON PREPROLACTIN MESSENGER RIBONUCLEIC-ACID SEQUENCES

被引：49

作者：

RYAN, R

SHUPNIK, MA

GORSKI, J

机构：

[1] UNIV WISCONSIN,DEPT BIOCHEM,MADISON,WI 53706

[2] UNIV WISCONSIN,DEPT ANIM SCI,MADISON,WI 53706

来源：

BIOCHEMISTRY | 1979年 / 18卷 / 10期

关键词：

D O I：

10.1021/bi00577a031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A complementary DNA (cDNA) probe has been synthesized for rat preprolactin messenger RNA (mRNA). Following preparative gel electrophoresis, the cDNA was characterized and estimated to be ~75% pure. The reverse transcript is estimated to be greater than 1000 nucleotides long and, therefore, is a full-length copy of the preprolactin mRNA. It is a faithful transcript, as evidenced by back-hybridization with the mRNA. By use of the cDNA as a hybridization probe, the levels of preprolactin mRNA in the pituitary of male and female rats were monitored and found to increase following estrogen treatment. Increases in preprolactin mRNA activity, assayed by in vitro translation in the wheat germ system, were paralleled by increases in hybridization to the cDNA probe after estrogen treatment. © 1979, American Chemical Society. All rights reserved.

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页码：2044 / 2048

页数：5

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