A MODEL PEPTIDE WITH ENHANCED HELICITY

被引:88
作者
MERUTKA, G [1 ]
SHALONGO, W [1 ]
STELLWAGEN, E [1 ]
机构
[1] UNIV IOWA,DEPT BIOCHEM,IOWA CITY,IA 52242
关键词
D O I
10.1021/bi00231a020
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The sequence of a model monomeric peptide, acetylA(EAAAK)3Aamide was altered to expedite measurement of peptide concentration and to enhance its fractional helical content. Replacement of the N-terminal alanine residue with a tryptophan residue provides a convenient chromophore for measurement of peptide concentration without diminishing the helical content. Replacement of the three lysine residues with arginine residues enhances the helical content without loss of their electrostatic contributions. Increasing the number of EAAAR sequence units in the peptide acetylW(EAAAR)(n)Aamide from three to five indicates that the spectral features anticipated for a completely helical peptide are closely approached.
引用
收藏
页码:4245 / 4248
页数:4
相关论文
共 22 条
[1]  
ARGOS P, 1982, INT J PEPT PROT RES, V19, P380
[2]   FAR ULTRAVIOLET ABSORPTION AND CIRCULAR-DICHROISM SPECTRA OF L-TRYPTOPHAN AND SOME DERIVATIVES [J].
AUER, HE .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1973, 95 (09) :3003-3011
[3]   DETERMINATION OF PROTEIN SECONDARY STRUCTURE IN SOLUTION BY VACUUM ULTRAVIOLET CIRCULAR-DICHROISM [J].
BRAHMS, S ;
BRAHMS, J .
JOURNAL OF MOLECULAR BIOLOGY, 1980, 138 (02) :149-178
[4]   CIRCULAR DICHROISM OF PUTATIVE UNORDERED POLYPEPTIDES AND PROTEINS [J].
DEARBORN, DG ;
WETLAUFER, DB .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1970, 39 (03) :314-+
[5]  
FASMAN GD, 1976, HDB BIOCH MOL BIOL, V1, P186
[6]   FOLDING OF A PEPTIDE CORRESPONDING TO THE ALPHA-HELIX IN BOVINE PANCREATIC TRYPSIN-INHIBITOR [J].
GOODMAN, EM ;
KIM, PS .
BIOCHEMISTRY, 1989, 28 (10) :4343-4347
[7]  
GRATZER WB, 1978, TECH PROTEIN ENZYM B, V108, P1
[8]   COMPUTED CIRCULAR DICHROISM SPECTRA FOR EVALUATION OF PROTEIN CONFORMATION [J].
GREENFIE.N ;
FASMAN, GD .
BIOCHEMISTRY, 1969, 8 (10) :4108-&
[9]   ULTRAVIOLET CIRCULAR DICHROISM OF POLYPEPTIDES [J].
HOLZWARTH, G ;
DOTY, P .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1965, 87 (02) :218-+
[10]  
HOUGHTEN RA, 1986, BIOTECHNIQUES, V4, P522