STRUCTURE OF UNDERIVATIZED BRANCHED OLIGOSACCHARIDES BY NEGATIVE-ION FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY

被引:29
作者
GAROZZO, D [1 ]
IMPALLOMENI, G [1 ]
MONTAUDO, G [1 ]
SPINA, E [1 ]
机构
[1] UNIV CATANIA,DIPARTIMENTO SCI CHIM,I-95125 CATANIA,ITALY
关键词
D O I
10.1002/rcm.1290060903
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We report here a study by negative-ion fast-atom bombardment (FAB) mass spectrometry of branched oligosaccharides containing a 3-O glycosidic linkage. Our results show that discrimination of the monosaccharide unit linked in the 3-O position is made possible by the analysis of the negative-ion FAB mass spectra. In the mass spectrum of A-trisaccharide (alpha-Fuc1-2-(alpha-GalNAc1-3)-Gal) the peak at m/z 307 can be explained as elimination of a neutral molecule of GaINAc (221 u) from the molecular ion. Also in the mass spectrum of 3-fucosyllactose (alpha-Fuc1-3-(beta-Gal1-4)-Glc) an intense peak at m/z 323, corresponding to the elimination of the fucose linked at the 3 position of glucose is present. To investigate further the fragmentations of the 3-O-linked sugars, the negative-ion FAB mass spectra of disaccharides have also been studied.
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页码:550 / 552
页数:3
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