SACCHAROMYCES-CEREVISIAE AND NEUROSPORA-CRASSA CONTAIN HEAVY-METAL SEQUESTERING PHYTOCHELATIN

被引：68

作者：

KNEER, R ^{[1
]}

KUTCHAN, TM ^{[1
]}

HOCHBERGER, A ^{[1
]}

ZENK, MH ^{[1
]}

机构：

[1] UNIV MUNICH,LEHRSTUHL PHARMAZEUT BIOL,KARLSTR 29,W-8000 MUNICH 2,GERMANY

来源：

ARCHIVES OF MICROBIOLOGY | 1992年 / 157卷 / 04期

关键词：

PHYTOCHELATIN; METALLOTHIONEIN; HEAVY METAL DETOXIFICATION; SACCHAROMYCES-CEREVISIAE; NEUROSPORA-CRASSA;

D O I：

10.1007/BF00248673

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In fungi, cellular resistance to heavy metal cytotoxicity is mediated either by binding of metal ions to proteins of the metallothionein type or by chelation to phytochelatin-peptides of the general formula (gamma-Glu-Cys)n-Gly. Hitherto, only one fungus, Candida glabrata has been shown to contain both metal inactivating systems. Here we show by unambiguous FAB-MS analysis that both a metallothionein-free mutant of Saccharomyces cerevisiae as well as a wildtype strain synthesize phytochelatin (PC2) upon exposure to 250-mu-M Cd2+ ions. The presence of Zn and/or Cu ions in the nutrient broth also induces PC2 synthesis in this organism. By Cd-109 exchange and subsequent monobromobimane fluorescence HPLC, it could be shown that the presence of Cd2+ in the growth medium also induces phytochelatin synthesis in Neurospora crassa, which contains metallothioneins.

引用

页码：305 / 310

页数：6

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