MOLECULAR-CLONING OF THE GENE ENCODING DEVELOPING SEED L-ASPARAGINASE FROM LUPINUS-ANGUSTIFOLIUS

被引：21

作者：

DICKSON, JMJJ

VINCZE, E

GRANT, MR

SMITH, LA

RODBER, KA

FARNDEN, KJF

REYNOLDS, PHS

机构：

[1] DSIR FRUIT & TREES,IMMUNOL & PLANT MOLEC BIOL UNIT,PALMERSTON NORTH,NEW ZEALAND

[2] UNIV OTAGO,DEPT BIOCHEM,DUNEDIN,NEW ZEALAND

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 20卷 / 02期

关键词：

L-ASPARAGINASE; DNA SEQUENCE; DEVELOPING SEED; LUPINUS-ANGUSTIFOLIUS (LUPIN);

D O I：

10.1007/BF00014503

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A genomic sequence encoding Lupinus angustifolius L-asparaginase has been obtained, and is the first report of this gene from a plant source. The 3.2 kb of DNA sequenced contains a 1136 bp 5' flanking sequence, four exons and three introns. Intron-exon borders were mapped by comparing the genomic sequence with that of a L. arboreus cDNA. Primer extension analysis revealed transcription start sites 16 bp and 13 bp 5' of the initiating ATG for L. angustifolius and L. arboreus, respectively. The 5' flanking region contained sequences associated with seed-specific expression.

引用

页码：333 / 336

页数：4

共 21 条

[1] ASPARAGINE METABOLISM - KEY TO NITROGEN NUTRITION OF DEVELOPING LEGUME SEEDS [J].