EVIDENCE FOR DIRECT INVOLVEMENT OF THE SARCOPLASMIC-RETICULUM CA2+-ATPASE IN A PASSIVE MONOVALENT CATION (K+/NA+) EXCHANGE

被引：3

作者：

DEJESUS, F ^{[1
]}

CUILLEL, M ^{[1
]}

DUPONT, Y ^{[1
]}

机构：

[1] CEN GRENOBLE,CEA,DEPT BIOL MOLEC & STRUCT,BIOPHYS MOLEC & CELLULAIRE LAB,CNRS,URA 520,GRENOBLE,FRANCE

来源：

FEBS LETTERS | 1995年 / 376卷 / 03期

关键词：

CA2+-ATPASE; SARCOPLASMIC RETICULUM; K+/NA+ EXCHANGE; THAPSIGARGIN; POTENTIAL SENSITIVE DYE; DISC(3)(5);

D O I：

10.1016/0014-5793(95)01267-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A specific inhibitor of SERCA-pumps, thapsigargin (TG) was used to demonstrate the direct involvement of the SR Ca2+-ATPase in passive K+/Na+ exchange. The K+-potential variations across vesicle membranes were measured in the absence of ATP with a fluorescent probe: 3,3'-dipropylthiodicarbocyanine iodide. Addition of EGTA dissipates the K+-potential whereas the presence of TG abolishes this effect. Our data prove that the Ca2+-ATPase translocates monovalent cations at a rate similar to the E(2)-->E(1) conformational change.

引用

页码：167 / 171

页数：5

共 27 条

[1] EVIDENCE FOR A CALCIUM-GATED CATION CHANNEL IN SARCOPLASMIC-RETICULUM VESICLES [J].