ELECTRONIC SORTING OF GRANULOCYTE PRECURSOR CELLS FROM STIMULATED BONE-MARROW

被引：5

作者：

GROGAN, WM

SCOTT, RB

COLLINS, JM

机构：

[1] VIRGINIA COMMONWEALTH UNIV,DEPT PATHOL,RICHMOND,VA 23298

[2] VIRGINIA COMMONWEALTH UNIV,DEPT MED,RICHMOND,VA 23298

[3] VIRGINIA COMMONWEALTH UNIV,CTR CANC,RICHMOND,VA 23298

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1979年 / 27卷 / 06期

关键词：

D O I：

10.1177/27.6.458137

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A commercial cell sorter was used to obtain preparations of cells in various stages of granulocyte development from rabbit marrows stimulated by inflammatory response. Marrow cells were fractionated on density gradients of Ficoll/Hypaque and each fraction sorted using light scatter. Trial and error selection of appropriate gradient fractions and light scatter windows allowed sorting or early (blast cells, promyelocytes), intermediate (myelocytes, metamyelocytes) and late stage (band cells, polys) granulocytes with enhanced purity.

引用

页码：1011 / 1016

页数：6

共 7 条

[1] GRANULOCYTOPOIESIS ( CONCLUDED ) [J].