SIMPLE METHOD FOR SEPARATING CELLS OF MICROCYSTIS-AERUGINOSA FOR COUNTING

被引:42
作者
HUMPHRIES, SE
WIDJAJA, F
机构
[1] Zoology Department, Australian National University, Canberra, 2600
来源
BRITISH PHYCOLOGICAL JOURNAL | 1979年 / 14卷 / 04期
关键词
D O I
10.1080/00071617900650331
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Heat was used to break up the mucilage-bound colonies of the blue-green alga, Microcystis aeruginosa Kiitz. emend. Elenkin, for cell counting. Samples were heated in a water bath at 80°C for 5-10 min, then agitated in a Vortex mixer for 30-60 s. The treatment separated cells completely and reaggregation of cells did not occur. There was no evidence that heating disintegrated the cells. © 1979 Taylor & Francis Group, LLC.
引用
收藏
页码:313 / 316
页数:4
相关论文
共 5 条
[1]  
Gorham PR, 1964, VERH INT VER LIMNOL, V15, P796, DOI DOI 10.1080/03680770.1962.11895606
[2]  
MAY V, 1978, Telopea, V1, P295
[3]   WATER-BLOOMS [J].
REYNOLDS, CS ;
WALSBY, AE .
BIOLOGICAL REVIEWS OF THE CAMBRIDGE PHILOSOPHICAL SOCIETY, 1975, 50 (04) :437-&
[4]   ENUMERATION OF NATURAL MICROCYSTIS POPULATIONS [J].
REYNOLDS, CS ;
JAWORSKI, GHM .
BRITISH PHYCOLOGICAL JOURNAL, 1978, 13 (03) :269-277
[5]  
Stein, 1973, HDB PHYCOLOGICAL MET, P289