GOLD LABELING OF RNA IN VIRUS-INDUCED MITOCHONDRIAL VESICLES IN THE SHEEP BLOWFLY LUCILIA-CUPRINA

被引：1

作者：

BINNINGTON, K

BROOKS, L

机构：

[1] Division of Entomology, Canberra A.C.T. 2601

来源：

TISSUE & CELL | 1992年 / 24卷 / 03期

关键词：

SS-RNA; DS-RNA; LUCILIA; BLOWFLY; VIRUS; MITOCHONDRIA; GOLD LABELING;

D O I：

10.1016/0040-8166(92)90057-E

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Membranous vesicles are thought to be the replication site for viral RNA of many plant and animal viruses. A relatively rare site of virus-associated vesiculation is that of the mitochondrial outer membrane. In this study, virus-induced mitochondrial vesicles of the blowfly, Lucilia cuprina, were labelled with ribonuclease/gold and an antibody against double stranded RNA (anti-polyinosinic:polycytidylic acid). Both methods showed the presence of RNA in the vesicles thus indicating they may be a site for viral RNA replication in Lucilia.

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收藏

页码：411 / 416

页数：6

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