CHEMICALLY MODIFIED RNA - APPROACHES AND APPLICATIONS

被引：91

作者：

HEIDENREICH, O ^{[1
]}

PIEKEN, W ^{[1
]}

ECKSTEIN, F ^{[1
]}

机构：

[1] MAX PLANCK INST EXPTL MED,HERMANN REIN STR 3,W-3400 GOTTINGEN,GERMANY

来源：

FASEB JOURNAL | 1993年 / 7卷 / 01期

关键词：

PHOSPHOROTHIOATE RNA; HAMMERHEAD RIBOZYMES; 2'-MODIFIED RNA;

D O I：

10.1096/fasebj.7.1.7678566

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The modification of phosphate into phosphorothioate internucleotidic linkages in various RNAs and their usefulness in identifying phosphate positions essential for function are described. Several modifications of the 2'-hydroxyl group of the ribose, particularly the replacement by fluorine atoms and amino groups, is discussed. These studies have been concentrated on hammerhead ribozymes in order to determine hydroxyl groups important for the catalytic activity. In addition these derivatives have been instrumental in rendering ribozymes more stable toward nucleases.

引用

页码：90 / 96

页数：7

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