KINETICS OF EXCHANGE PROCESSES IN THE ADSORPTION OF PROTEINS ON SOLID-SURFACES

被引：40

作者：

BALL, V

HUETZ, P

ELAISSARI, A

CAZENAVE, JP

VOEGEL, JC

SCHAAF, P

机构：

[1] UNIV STRASBOURG 1,INST CHARLES SADRON,CNRS,F-67083 STRASBOURG,FRANCE

[2] INSERM,CTR RECH ODONTOL,F-67000 STRASBOURG,FRANCE

[3] ECOLE NORMALE SUPER LYON,CNRS,UNITE BIOMERIEUX,F-69364 LYON 07,FRANCE

[4] ECOLE EUROPEENNE HAUTES ETUD IND CHIM STRASBOURG,CNRS,URA 405,F-67008 STRASBOURG,FRANCE

[5] CTR REG TRANSFUS SANGUINE,INSERM,U311,F-67085 STRASBOURG,FRANCE

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 15期

关键词：

HOMOMOLECULAR EXCHANGE;

D O I：

10.1073/pnas.91.15.7330

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The homogeneous exchange process whereby IgG molecules adsorbed onto latex particles are replaced by IgG molecules from the bulk solution was studied by means of 125I radiolabeling. The exchange mechanism was investigated on surfaces saturated with either labeled or unlabeled proteins in the presence of a solution of the opposite species in two sets of independent experiments. After rinsing of the surface by pure buffer followed by supplementary IgG adsorption, the exchange process followed a kinetic law of first order with respect to the IgG molecules from the bulk solution, and the apparent exchange rate constant was (2.3 +/- 0.4) x 10(-5) cm.hr(-1).

引用

页码：7330 / 7334

页数：5

共 16 条

[1] INTERACTION OF FIBRINOGEN WITH SOLID-SURFACES OF VARYING CHARGE AND HYDROPHOBIC HYDROPHILIC BALANCE .2. DYNAMIC EXCHANGE BETWEEN SURFACE AND SOLUTION MOLECULES [J].