AUTORADIOGRAPHIC LOCALIZATION OF NK1 AND NK3 TACHYKININ RECEPTORS IN RAT-KIDNEY

The distribution of neurokinin receptors in rat kidney, renal artery, renal vein, and proximal ureter was evaluated by autoradiography after in vitro labeling of NK1 sites with [I-125]Bolton-Hunter substance P (BHSP) or NK3 sites with [I-125][MePhe(7)]neurokinin B ([MePhe7]NKB). Film autoradiography using [I-125][MePhe(7)]NKB revealed specific binding sites associated with the renal vein and its large branches, the renal pelvis, the inner strip of outer renal medulla, and the proximal ureter. High-resolution autoradiograms demonstrated that these sites were localized to the smooth muscle layer in the veins, pelvis, and ureter. Neither the renal arterial system nor the renal cortex contained specific [I-125][MePhe(7)]NKB binding sites although a high level of nonspecific binding was associated with the renal artery. Specific binding of [I-125]BHSP was associated with the renal artery and renal pelvis but not the renal veins. Arterial NK1 receptors appeared to be localized to the adventitia. The results indicate that at least two types of tachykinin receptor are present in the rat kidney. The distinct localization observed for most of the NK1 and NK3 receptors suggests that they have different functions.